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[Spencer from The Hills]

The trailers and the website played up the game's five factions and the demo showed directions to start quests with four of them, with a Tremere errand being played. To me, it looked like the player character would be able to start affiliating with factions fairly early....

[Spencer from The Hills]

CotBG also refers to them:
"Boa constrictors are native to the Americas, with Ministers identifying as members of the Tlacique — a vampire cult focused on Aztec mythology — commonly taking constrictor form using the Discipline of Protean."
Without the context of previous editions, this could mean that some Tlacique are Ministers and some aren't, which would be to say that it isn't a bloodline....